NHLBI CLINICAL INVESTIGATOR AWARD PROGRAM

Project: Research project

Description

Post-transfusion hepatitis remains a major medical problem in spite of
screening for hepatitis B surface antigen in the blood of prospective
donors. Approximately ten percent of post-transfusion hepatitis is due to
HBV, the remaineder is mainly due to non A non B hepatitis. Both heaptitis
B and non A non B heaptitis can cause chronic heaptitis and epidemiological
studies have suggested a casual relationship between HBV and heaptocellular
carcinoma. Integration of HBV DNA in productively infected liver (acute
and chronic) and hepatocellular carcinoma will be analyzed by 1) cloning
HindIII digested DNA fragments which contain viral sequences but are larger
than viral DNA, 2) determining whether the cloned fragments contain HBV DNA
linked to cell DNA, 3) identifying the junction sites of intergration in
viral and cellular DNA by sequencing, 4) assessing the orientation of the
integrated viral DNA,. and 5) assessing the ability of cloned integrated
DNA graments to transform cultured cells. Particles resembling
picornaviruses have been found in the serum and liver of chimpanzees with
on A non B hepatitis. A non A non B hepatitis virus will be investigated
by: 1) growing the virus in tissue culture, 2) identifying and
radioactively labeling the viral nucleic acid, and 3) clining the viral
nucleic acid in E. coli. The cloning of viral nucleic acid will permit:
a) the development of a DNA probe to assay for the virus in blood, tissue,
and tissue culture and b) an attempt to express viral genes in E. coli. A
probe may be used to investigate the molecular biology, epidemiology, host
range and course of infection of the virus. Viral antigens synthesized in
E. coli will be used for the development of a serological screening test
for the virus.
StatusFinished
Effective start/end date5/1/856/30/87

Funding

  • National Institutes of Health
  • National Institutes of Health

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National Heart, Lung, and Blood Institute (U.S.)
Viral DNA
Research Personnel
Viruses
Hepatitis B
Nucleic Acids
Hepatitis
DNA
Escherichia coli
Pan troglodytes
Molecular Epidemiology
Viral Genes
Viral Antigens
Liver
DNA Probes
Virus Diseases
Hepatitis B Surface Antigens
DNA Sequence Analysis
Hepatitis B virus
Organism Cloning

ASJC

  • Medicine(all)