MODEL OF LYME DISEASE

Project: Research project

Description

The proposed research will develop and characterized a recently
discovered rat model of Lyme disease; the first reproducible
animal model of this important human illness. When LEW/N rats
are inoculated with Borrelia burgdorferi, the causative agent of
Lyme disease, they uniformly develop multisystemic infection and
arthritis, the latter lesion being the most common and serious
sequela of Lyme disease in man. Experiments described here will
examine in inoculated rats, optimal conditions for inducing
disease, the pathogenesis and duration of arthritis, immunological
defenses during infection, whether other manifestations of Lyme
disease occur, such as cutaneous, myoardial and neurological
involvement, the virulence of B. burgdorferi obtained from
different parts of the world, and the effect of genotype of
susceptibility to disease. Studies will utilize a variety of
techniques including: microbiological culture, histopathology, and
immunohistochemistry to identify inflammatory cells, in vitro
mitogenesis assays for cellular immunity, serological tests for
antibody to B. burgdorferi and adoptive immunization. The role
of T cells in lesion development and host defenses will require the
use of athymic rats. Apart from the benefits these studies will
have for Lyme disease research, they also after offer an
opportunity to study a human-related arthritis for which the
etiology is known.
StatusFinished
Effective start/end date7/1/8811/30/12

Funding

  • National Institutes of Health
  • National Institutes of Health: $372,438.00
  • National Institutes of Health
  • National Institutes of Health
  • National Institutes of Health: $372,438.00
  • National Institutes of Health: $333,656.00
  • National Institutes of Health
  • National Institutes of Health
  • National Institutes of Health: $590,849.00
  • National Institutes of Health: $261,268.00
  • National Institutes of Health: $380,000.00
  • National Institutes of Health: $334,125.00
  • National Institutes of Health: $228,448.00
  • National Institutes of Health
  • National Institutes of Health: $334,125.00
  • National Institutes of Health: $334,125.00
  • National Institutes of Health: $250,241.00
  • National Institutes of Health
  • National Institutes of Health: $326,274.00
  • National Institutes of Health
  • National Institutes of Health: $456,554.00
  • National Institutes of Health
  • National Institutes of Health: $269,105.00
  • National Institutes of Health: $239,215.00

Fingerprint

Lyme Disease
Borrelia burgdorferi
Spirochaetales
Arthritis
Immune Sera
Infection
Antigens
Immunity
Borrelia Infections
Myocarditis
Nude Rats
Decorin
Genotype
Antibodies
Gene Expression
Recombinant Proteins
Tick-Borne Diseases
Staphylococcal Protein A
Serologic Tests
Unnecessary Procedures

ASJC

  • Medicine(all)
  • Immunology and Microbiology(all)