MICROANALYSIS OF RAPIDLY-FROZEN TISSUE IN THE FIELD EMISSION STEM

Project: Research project

Project Details

Description

Measurements on thin hydrated cryosections show that x-ray microanalysis
is limited to areas greater than l-mu-m2 and to the major elements because
of radiation damage; the maximum allowed doses are only approximately 104
e/nm2 for analysis of hydrated sections. For freeze-dried cryosections
low-dose STEM micrographs show good structural preservation down to the
level of single membranes. The small probe size and high stability of the
low temperature stage in the HB501 STEM facilitates high resolution
analysis of 10-nm scale structures in such sections, although certain
detail is lost on irradiation. The sensitivity for microanalysis of
elements such as calcium in small organelles is in the range of a few tens
of atoms.
StatusFinished
Effective start/end date3/1/972/28/98

Funding

  • National Institutes of Health: $115,000.00

ASJC

  • Medicine(all)

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