MECHANISTIC STUDIES OF GENETIC RECOMBINATION

  • Kowalczykowski, Stephen C, (PI)

Project: Research project

Description

The long-term objective of this research proposal is to contribute to the
biochemical understanding of the mechanism of homologous recombination.
The general approach is to enzymatically characterize the purified proteins
known to participate in genetic recombination in E. coli and to determine
their enzymatic mechanism when acting individually or as component of a
more complex system. These proteins include the recA, SSB, and recBCD
proteins of E. coli. The specific approach is to pursue physical-biochemical, enzymatic, and
structure-functional characterization of a protein that plays a central
role in genetic recombination, the recA protein of E. coli. To assess the
biological significance of the many recA protein activities and to
determine how they contribute to enzymatic function, the biochemical
properties of mutant recA proteins will be examined. Finally, in order to
better understand how the activities of the recA protein are coordinated
with those of SSB and recBCD enzymes to promote the homologous pairing, in
vitro assays that require the activities of these proteins will be examined
mechanistically. Understanding the molecular mechanism of genetic recombination should shed
light aberrant on biological events such as chromosomal translocations and
error-prone repair and should contribute to the fundamental biochemical
knowledge required to develop useful gene replacement therapies based on
homologous recombination.
StatusFinished
Effective start/end date4/1/823/31/00

Funding

  • National Institutes of Health
  • National Institutes of Health
  • National Institutes of Health
  • National Institutes of Health
  • National Institutes of Health
  • National Institutes of Health
  • National Institutes of Health
  • National Institutes of Health
  • National Institutes of Health
  • National Institutes of Health
  • National Institutes of Health
  • National Institutes of Health
  • National Institutes of Health: $49,137.00
  • National Institutes of Health
  • National Institutes of Health
  • National Institutes of Health

Fingerprint

genetic recombination
Rec A Recombinases
Genetic Recombination
Proteins
proteins
DNA
Escherichia coli
DNA Helicases
Homologous Recombination
RecQ Helicases
Escherichia coli Proteins
homologous recombination
Sequence Homology
Biological Phenomena
Recombinant DNA
Nucleic Acids
mutants
therapeutics
Genetic Translocation
Research

ASJC

  • Medicine(all)
  • Immunology and Microbiology(all)