MECHANISM OF ACTION OF THE V-ERB A ONCOGENE OF AEV

  • Privalsky, Martin L (PI)

Project: Research project

Project Details

Description

The v-erb A oncogene of avian erythroblastosis virus potentiates erythroid
oncogenic transformation and alters the growth properties of fibroblasts.
The v-erb A locus is a virally-transduced and altered copy of a cellular
gene for a thyroid hormone receptor. We wish to understand the mechanism
of action of the V-erb A oncogene, and to relate its role in virus-induced
neoplasias to events in normal cell differentiation and in human cancers. A. The molecular basis behind the activation of the thyroid hormone
receptor locus into the v-erb A oncogene will be elucidated. The
biochemical differences between the v- and c-erb A proteins will be
elucidated and related to the differing oncogenic and transcriptional
properties of these two different polypeptides. The role of
transcriptional repression by v-erb A in mediating the transformed cell
phenotype will be determined, and possible additional mechanisms of action
of v-erb A in the neoplastic cell will be investigated. B. The targets of v-erb A action in establishing the transformed state
will be characterized. Some evidence suggests that thyroid hormone
responsive genes are not the actual physiological targets of v-erb A action
in the neoplastic cell; the true targets will be identified. The
structural and biological significance of the differing DNA binding
properties of the v- and c-erb A proteins will be determined. The nature
of cell factors apparently involved in conferring specific DNA binding on
the v-erb A protein, and their relationship to similar factors elucidated
for the c-erb A protein, will be elucidated. C. The role of thyroid hormone receptors in Xenopus metamorphosis will be
determined. The different types of thyroid hormone receptor and receptor-
like proteins encoded in the Xenopus genome will be elucidated, and the
roles these different gene products play in the tissue specific, temporally
regulated process of metamorphosis will be investigated.
StatusFinished
Effective start/end date12/1/9010/31/14

Funding

  • National Institutes of Health: $281,106.00
  • National Institutes of Health: $215,948.00
  • National Institutes of Health
  • National Institutes of Health
  • National Institutes of Health: $300,645.00
  • National Institutes of Health: $46,043.00
  • National Institutes of Health: $247,514.00
  • National Institutes of Health: $275,220.00
  • National Institutes of Health
  • National Institutes of Health
  • National Institutes of Health: $59,139.00
  • National Institutes of Health: $276,347.00
  • National Institutes of Health: $291,625.00
  • National Institutes of Health
  • National Institutes of Health
  • National Institutes of Health: $272,954.00
  • National Institutes of Health
  • National Institutes of Health
  • National Institutes of Health: $291,625.00
  • National Institutes of Health: $300,645.00
  • National Institutes of Health
  • National Institutes of Health
  • National Institutes of Health: $300,645.00
  • National Institutes of Health
  • National Institutes of Health: $236,369.00

ASJC

  • Medicine(all)

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