IMMUNOCHEMISTRY--IMMUNOASSAYS TO ARTIFICIAL ENZYMES

  • Kurth, Mark J, (PI)

Project: Research project

Description

CANDIDATE: Dr. Mark J. Kurth's Ph.D., postdoctoral, and early
professional work (six years at the University of California/Davis)
establish him as a successful independent investigator in synthetic
organic chemistry, especially in the area of enantioselective
methodology development and natural product total synthesis. His
immediate career goal is to become an expert in immunochemistry,
particularly as it applies to environmental assessment and
toxicology. Toward that goal, Dr. Kurth has three environmental
health projects (NIH, USDA, and FDA funded) under collaborative
investigation (with Dr. M.E. Mount, Veterinary Medicine, U.C. Davis
and Dr. H.G. Hammock, Entomology, U.C. Davis) and two projects
pending review (NIEHS Superfund and EPA proposals). His long-term
career goals are three-fold: (i) development of immunochemical
methods for the detection/quantification of toxic substances in the
environment, (ii) application of immunochemical methods to the
study of mechanism of and site of toxic action of hazardous
substances, and (iii) fundamental research aimed at generating
artificial enzymes (abzymes) for application in toxicology and
environmental health sciences by combining the synthetic expertise
required to prepare transition-state mimics and the immunochemical
expertise required to develop antibodies capable of 'enzymatic
action.' A RESEARCH CAREER DEVELOPMENT AWARD would tremendously
enhance Dr. Kurth's opportunity to accomplish the career objectives
delineated in this proposal in two critical ways. First, it will
relieve him of a myriad of teaching and committee responsibilities,
thus freeing him to focus his energy and efforts on research.
Second, it will provide him with the flexibility and opportunity
to personally work in leading toxicology and bioorganic chemistry
laboratories. ENVIRONMENT: Pending the success of this
application, Dr. Kurth has identified five laboratories with which
he will be associate as a visiting scholar. As documented in
Appendices III-IX, these include: Dr. Jean-Pierre Mach (Institute
of Biochemistry, University of Lausanne), Dr. Robert Scibienski
(Molecular Biology, U.C. Davis) Dr. Alan Buckpitt (Veterinary
Pharmacology and Toxicology, U.C. Davis), Dr. Paul Barlett
(Chemistry, U.C. Berkeley), and Dr. Alex Karu (Hybridoma
Laboratory, U.C. Berkeley). Dr. Kurth's career goals are very much
in line with the Chemistry Department's expansion plans in the
'chemistry of biological macromolecules' area. An outstanding
research infrastructure in the areas of chemistry, biochemical
toxicology, and bioorganic chemistry exists on the U.C. Davis
campus which will provide Dr. Kurth with ample opportunity for
scientific interaction and will significantly enhance the
development of his career. RESEARCH: Herein Dr. Kurth proposes
applying synthetic organic and immunochemistry to environmental
health research addressing three important objectives: (I)
development of immunochemical methods for the
detection/quantification of toxic substances in the environment,
(II) application of immunochemical methods to the study of
mechanism of and site of toxic action of hazardous substances, and
(III) fundamental research aimed at generating artificial enzymes
(abzymes) for application in toxicology and environmental health
sciences by combinging the synthetic expertise required to prepare
transition-state mimics and the immunochemical expertise required
to develop antibodies capable of 'enzymatic acton.' While
objective I constitutes a major portion of this proposal, its
realization will provide entry to objective II. In turn, objective
III is a logical extension of I and II and sets a longer term goal
of Dr. Kurth's research program.
StatusFinished
Effective start/end date12/5/886/30/94

Funding

  • National Institutes of Health
  • National Institutes of Health
  • National Institutes of Health
  • National Institutes of Health
  • National Institutes of Health

Fingerprint

Immunochemistry
Immunoassay
Enzymes
Research
Toxicology
Toxic Actions
Poisons
National Institute of Environmental Health Sciences (U.S.)
Entomology
Ecotoxicology
Hazardous Substances
United States Department of Agriculture
Veterinary Medicine
Antibodies
Biological Products
Biochemistry
Molecular Biology
Teaching
Research Personnel
Health

ASJC

  • Environmental Science(all)
  • Medicine(all)