IMMUNOASSAYS FOR PYRROLIZIDINE ALKALOIDS AND METABOLITES

We propose to investigate effects of 2,3,7,8-tetrachlorodibenzo-p-dioxin
(TCDD) on the motor (central and peripheral) system in several species of
rodents by means of a broadbased, multidisciplinary approach involving
behavior, neurochemistry, neurophysiology, and neuropathology. We will
screen several species of laboratory rodents for neurotoxic effects of TCDD
to develop an appropriate animal model or to rule out neurotoxicity of
TCDD; we will assess whether TCDD alters the ability of the neuromuscular
system to repair itself following injury, and whether traumatic damage to
the neuromuscular system increases its sensitivity to TCDD; and, we will
investigate mechanisms of neurotoxic action of TCDD on the central nervous
system and neuromuscular system in a susceptible rodent species. The
results of these studies may be expected to contribute valuable information
concerning this potentially major public health hazard.