GENETIC ENGINEERING APPROACHES FOR AIDS VACCINES

Project: Research project

Project Details

Description

We have molecularly cloned (1), sequenced and expressed (2) the genes of
the retrovirus associated with Acquired Immune Deficiency Syndrome (AIDS),
and we have initiated genetic engineering approaches for the development
and testing of subunit viral vaccines. The predicted sequence of the viral
envelope (env) protein has been used to design synthetic peptides, and the
entire env gene and various portions are to be cloned into bacterial,
yeast, and mammalian expression vectors. Purified env polypeptides
produced in these expression systems and synthetic peptides will be used to
raise polyclonal antibodies in rabbits and subhuman primates and to raise
monoclonal antibodies of mouse and human origin. Various adjuvants and
antigen-presentation methods will be tested. Viral-specific antibodies
will be evaluated in virus neutralization assays and in cytotoxicity
assays. Cell-mediated immune responses will be assessed in sub-human
primates. The form(s) of viral env antigen giving rise to the strongest
and most durable measured immune responses will be used to immunize
chimpanzees; these animals and cohort controls will be challenged with AIDS
virus and protection from infection and disease will be assessed.
StatusFinished
Effective start/end date8/1/8512/31/89

Funding

  • National Institutes of Health

ASJC

  • Medicine(all)
  • Immunology and Microbiology(all)

Fingerprint Explore the research topics touched on by this project. These labels are generated based on the underlying awards/grants. Together they form a unique fingerprint.