Project: Research project

Project Details


This proposal is designed to refine the understanding of gene linkage
relationships in the mouse and allow precise comparative mapping analyses
with the human genome. DNA obtained from a large series of
[(C3H/HeJ-gld/gld x Mus spretus) F1 X C3H/HeJ-gld/gld] interspecific
backcross mice will be used to identify genetic markers at interval of
between 10 to 20 centi-Morgans throughout the genome. As this segregation
map develops it will be utilized to identify and define the extent and
border of conserved linkage groups common to mice and humans. Detailed
analysis of mouse chromosome 1 genetics is the central focus of the
proposal. The goals include: i) creation of a distal mouse chromosome 1
specific library; ii) detailed comparative mapping with syntenic groups on
human chromosomes 1q, 2q and possibly 18q; iii) physical mapping studies to
generate a molecular map of distal mouse chromosome 1 to complement the
detailed genetic map and iv) comparative physical mapping of different Mus
species and areas of the human genome. The large number of interspecific
backcross mice (over 1200 available) combined with the generation of large
numbers of markers will allow the resolution of a 0.5 cm genetic map of
distal mouse chromosome 1. The refinement of the mouse genomic map at both
a genetic and physical level will allow better analyses of the
relationships between mouse genetic models for disease and their possible
human counterparts as well as fundamental structure/function correlations
concerning genomic organization. The studies should provide important
insight into aspects of mammalian meiotic recombination and the evolution
of mammalian genomes.
Effective start/end date12/6/89 → …


  • National Institutes of Health: $164,008.00
  • National Institutes of Health
  • National Institutes of Health: $288,025.00


  • Medicine(all)
  • Biochemistry, Genetics and Molecular Biology(all)


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