Generation of a TCR transgenic mouse to study Chlamydia genital tract infection

Project: Research project

Description

? DESCRIPTION (provided by applicant): Chlamydia infections are a major cause of sexually transmitted disease in the US. Greater understanding of protective immunity to Chlamydia at mucosal surfaces is urgently required if an effective vaccine is to become a reality. This application proposes to develop a TCR transgenic mouse to uncover fundamental aspects of mucosal CD4 T cell biology during infection of the reproductive tract with Chlamydia. Our experimental approach is unique in that it will utilize a natural route of infection, an establishe mouse model of infection where CD4 T cells participate in pathogen clearance, and involve direct visualization of pathogen-specific CD4 T cells using reagents and techniques that we will develop. Our application specifically proposes to, (i) identify antigen presenting cell subsets tha drive CD4 T effector cell priming in the reproductive tract, and (ii) define the heterogeneity of te CD4 T cell memory pool required for protective immunity in the upper reproductive tract. Understanding both these issues will be vitally important for the generation of a vaccine against Chlamydia, one of the most important pathogens affecting the reproductive health of young women in the US.
StatusFinished
Effective start/end date2/1/151/31/17

Funding

  • National Institutes of Health: $230,192.00
  • National Institutes of Health: $191,608.00

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Reproductive Tract Infections
Chlamydia Infections
Transgenic Mice
Chlamydia
T-Lymphocytes
Immunity
Vaccines
Reproductive Health
Antigen-Presenting Cells
Sexually Transmitted Diseases
Infection
Cell Biology

Keywords

  • Medicine(all)
  • Immunology and Microbiology(all)