FOURIER TRANSFORM MASS SPECTROMETRY OF OLIGOSACCHARIDES

Project: Research project

Project Details

Description

The broad long term goal of this research is to develop a new mass
spectrometric method for the structural elucidation of oligosaccharides.
Oligosaccharides (OS) play a central role in mammalian biology in many
fundamental processes such as cell-cell recognition, adhesiveness,
control of cell division, cellular differentiation and malignant
transformation. Hence, in the study of cancer and other related
diseases, a general, sensitive, and accurate method of analysis is
clearly desired. Mass spectrometry has the potential to offer many of
these features. Our immediate goal is to understand the production and unimolecular
chemistry of complex gas-phase oligosaccharide ions. The newly developed
external source Fourier transform mass spectrometry instrument will be
utilized and further developed for the specific analysis of these
compounds. This method is unique and allows ions to be produced and
trapped for long periods of time. The addition of the external source
has allowed the use of fast atom bombardment to generate gas-phase
bio-organic ions. Preliminary experiments have been performed showing
major differences between this technique and other mass spectrometric
methods. We find that matrix interference in the spectra is minimal add
substantial fragmentation is obtained. This is due to the relatively
longer detection time scale of the instrument. During the detection
period, slow metastable decay occurs and allows oligosaccharide ions to
undergo fragmentation. Matrix cluster ions also undergo metastable
decomposition. However, these ions fragment more quickly due to the loose
nature of the bonding. The result is a spectrum of oligosaccharide ions
in the near absence of matrix interference. Studies are proposed to gain
a better understanding of the unimolecular decay. In addition,
conditions will be obtained to optimize structural information from
FAB/FTMS alone (i.e. without further tandem MS determinations). This
would make powerful the coupling of liquid chromatography techniques such
as flow FAB (and electrospray) to this mass spectral method to analyze
complex oligosaccharide mixtures. Direct application to glycolipids and
high-mannose compounds will be performed through a collaboration with a
natural products chemist and an oligosaccharide biochemists.
StatusFinished
Effective start/end date5/1/931/31/16

Funding

  • National Institutes of Health: $272,543.00
  • National Institutes of Health: $187,269.00
  • National Institutes of Health: $323,729.00
  • National Institutes of Health: $236,688.00
  • National Institutes of Health: $254,167.00
  • National Institutes of Health: $148,316.00
  • National Institutes of Health
  • National Institutes of Health: $241,450.00
  • National Institutes of Health
  • National Institutes of Health
  • National Institutes of Health: $152,680.00
  • National Institutes of Health: $229,286.00
  • National Institutes of Health: $236,044.00
  • National Institutes of Health
  • National Institutes of Health: $28,194.00
  • National Institutes of Health: $167,511.00
  • National Institutes of Health: $34,850.00
  • National Institutes of Health
  • National Institutes of Health
  • National Institutes of Health: $45,535.00
  • National Institutes of Health: $254,725.00
  • National Institutes of Health: $212,421.00
  • National Institutes of Health: $157,175.00
  • National Institutes of Health: $265,248.00
  • National Institutes of Health: $270,795.00

ASJC

  • Medicine(all)
  • Biochemistry, Genetics and Molecular Biology(all)

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