DEVELOP METHODS OF ANALYSIS OF HUMAN COLOSTRUM AND MILK

  • Lonnerdal, Bo, (PI)

Project: Research project

Description

The non-protein nitrogen portion of human milk represents approximately 25%
of the total nitrogen containing compounds present in human milk. This
fraction contains all of the newly discovered growth factors and other
biologically functional components of these important fluids. This project
will focus in two areas: 1) A longitudinal study will be performed to analyze known whey proteins by
immunological methods. Glycoproteins will be isolated as a group, then
separated into individual components and quantitated. Metalloproteins will
be isolated in groups, separated and quantitated. Vitamin-binding proteins
will be quantitated. Unknown whey proteins will be isolated, identified
and quantitated. 2) Methods for isolating NPN compounds will be developed and evaluated.
Post-prandial, diurnal and longitudinal variations in known individual NPN
compounds will be determined. Unknown NPN compounds will be separated by
chromatographic techniques, quantitated with regard to nitrogen content and
characterized.
StatusFinished
Effective start/end date2/1/862/28/89

Funding

  • National Institutes of Health
  • National Institutes of Health
  • National Institutes of Health
  • National Institutes of Health
  • National Institutes of Health
  • National Institutes of Health

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Colostrum
Human Milk
Nitrogen
Metalloproteins
Nitrogen Compounds
Vitamins
Longitudinal Studies
Meals
Intercellular Signaling Peptides and Proteins
Glycoproteins
Whey Proteins

ASJC

  • Medicine(all)