BIOCHEMICAL MODULATION OF RETINAL GLIOSIS

Project: Research project

Description

The migration of retinal glial cells to extra-retinal sites is an important
aspect of the pathophysiology of many proliferative retinopathies including
proliferative vitreoretinopathy (PVR), and fibovascular-retinopathies such
as diabetic vitreoretinopathy, retinopathy of prematurity, and traumatic
vitreoretinopathy. The most attractive hypothesis which accounts for
gliosis as a final common pathway in these pathologies is the secretion or
release of a protein by the retina which stimulates the migration and
growth of retinal glia under a variety of physiological and pathological
stresses. We have purified a protein from normal bovine retina which
elicites a migratory response in various neural glia, which we have named
the retinal chemotactic protein. The research proposed here will provide an accurate determination of the
physical properties of the retinal chemotactic protein and measure its
effect on the migration, proliferation, and deposition of extracellular
matrix by retinal glia in vitro. Studies of ocular cells in vitro will
determine the physiological parameters regulating the release or secretion
of this protein. This work will further establish the role of the retinal
chemotactic protein in retinal detachment using the cat animal model.
Finally, vitreous aspirates and celullar membranes removed during
vitrectomy will be used to establish the role of this same protein in human
pathologies involving glial migration and proliferation. This work will provide a basic understanding of the retina's response to
injury. Since proliferated glial cells directly interfere with retinal
reattachment, and may provide a structure upon which other ocular cell
types may attach and divide, these results should lead to an enhanced
ability to design new forms of treatment for these devastating retinal
diseases.
StatusActive
Effective start/end date7/1/86 → …

Funding

  • National Institutes of Health: $366,900.00
  • National Institutes of Health
  • National Institutes of Health
  • National Institutes of Health
  • National Institutes of Health
  • National Institutes of Health
  • National Institutes of Health: $356,420.00
  • National Institutes of Health
  • National Institutes of Health
  • National Institutes of Health
  • National Institutes of Health: $365,694.00
  • National Institutes of Health
  • National Institutes of Health
  • National Institutes of Health
  • National Institutes of Health
  • National Institutes of Health
  • National Institutes of Health

Fingerprint

Retinal Detachment
Neuroglia
Gliosis
Fibroblast Growth Factor Receptors
Retina
Proteins
Retinal Pigment Epithelium
Gene Expression
Fibroblast Growth Factor 2
Oxidation-Reduction
Retinal Neurons
Oxidative Stress
Organized Financing
Cell Adhesion Molecules
Retinopathy of Prematurity
Diabetic Retinopathy
Cats
Animal Models
Pathology
Macular Degeneration

ASJC

  • Medicine(all)