BIOCHEMICAL MECHANISM AND FUNCTION OF DNA HELICASES

  • Kowalczykowski, Stephen C (PI)

Project: Research project

Project Details

Description

The studies described in this research proposal are directed at
determining the biochemical mechanism and biological function of
DNA helicases. The proteins to be investigated include the E. coli
recBCD enzyme, rep protein, and uvrD protein. These proteins play
important roles in the processes of DNA replication, recombination
and DNA repair. The biochemical mechanism of helicase action will
be investigated by taking advantage of a rapid, continuous, and
accurate fluorescence helicase assay for DNA unwinding that has
been recently developed in my laboratory. This assay poses several
advantages over traditional methods and will be used to
biochemically characterize the helicase activities of the recBCD
enzyme, rep protein and uvrD protein. In addition, the
relationship of the ATPase activity of these helicases to their DNA
unwinding activity will be examined. To better understand the
physical basis of the DNA unwinding activity, the equilibrium DNA
and ATP binding properties of these protein will be examined. All
of these studies will also be extended to a characterization of the
biochemical properties of mutant DNA helicases in order to attempt
to relate the biochemical activities with biological function.
StatusFinished
Effective start/end date12/1/885/31/20

Funding

  • National Institutes of Health
  • National Institutes of Health: $480,653.00
  • National Institutes of Health
  • National Institutes of Health
  • National Institutes of Health: $431,160.00
  • National Institutes of Health
  • National Institutes of Health
  • National Institutes of Health
  • National Institutes of Health
  • National Institutes of Health: $478,885.00
  • National Institutes of Health: $486,678.00
  • National Institutes of Health: $111,375.00
  • National Institutes of Health: $286,848.00
  • National Institutes of Health: $416,219.00
  • National Institutes of Health: $127,178.00
  • National Institutes of Health: $418,788.00
  • National Institutes of Health: $505,216.00
  • National Institutes of Health: $438,885.00
  • National Institutes of Health: $432,770.00
  • National Institutes of Health: $473,980.00
  • National Institutes of Health: $471,024.00
  • National Institutes of Health
  • National Institutes of Health
  • National Institutes of Health: $99,697.00
  • National Institutes of Health
  • National Institutes of Health: $295,362.00
  • National Institutes of Health: $476,186.00
  • National Institutes of Health: $471,697.00
  • National Institutes of Health

ASJC

  • Medicine(all)
  • Biochemistry, Genetics and Molecular Biology(all)

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