Project: Research project

Project Details


DESCRIPTION: This application requests funds to
purchase a spotter, reader and associated software for establishing a DNA
microarray center at UC Davis. The center will provide access for 18 NIH funded
investigators to the technology and equipment necessary for generating custom
DNA chips. In addition, three centers (Superfund, the NIEHS Center for
Agricultural Chemicals and the California Primate Research Center) and students
in a number of NIH supported training programs (training grants in
Environmental Toxicology, Environmental Pathology, Pulmonary Medicine) will
benefit from access to this equipment. As EST sets are established and
accessibility of the expertise and equipment needed become greater, we
anticipate that there will be more users than listed in the current
The recent introduction of high throughput techniques for genome wide
expression analysis has opened a number of opportunities to understand the
variety and complexity of cellular responses to various manipulations. In
addition, these approaches have allowed a rapid and more detailed view of
alterations in gene expression patterns in response to diseases, which in turn
has greatly accelerated the identification of potential drug targets for the
treatment of these diseases. While a number of research questions can be
answered by the use of DNA arrays, access to the equipment and the cost of
commercial arrays has hampered full application of these approaches by many
investigators. Much of the work outlined by the major users in this application
focuses on changes in target tissues/cells including cells of the pulmonary,
reproductive, cardiovascular, neurologic and dermatologic systems in response
to a diverse set of agents. In all of these studies the intent is to identify
changes, which occur in gene regulation in response to the toxicant, and as a
lead toward understanding the critical events leading to toxicity. A second
group of investigators will apply these approaches to study of alterations in
response to diseases including schizophrenia as well as those associated with
susceptibility to viral infections. In all cases the ready availability of
flexible technology for generation of sufficient numbers of chips to allow
detailed, thorough analysis of changes occurring in transcriptional regulation
will both broaden the questions that can be posed by this group of
investigators and will increase the speed with which they can be answered.
Effective start/end date6/1/015/31/02


  • National Institutes of Health: $140,000.00


  • Medicine(all)


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